Fig. 2
Effects of IL2-GMCSF on DC 2.4 cell bioactivities. a FD40 engulfment and b cell proliferation of DC2.4 cells under different dosage of IL2-GMCSF or cytokines; c–d maturation of DC 2.4 cells with different cytokine treatments were indicated by expression of CD80/CD86 (e) and MHC-II/CD83 (f) detected by flow cytometry. e IL-12 and (F) MDC secretion by DC 2.4 cells treated with IL2-GMCSF or GM-CSF were assayed with ELISA. g Western blotting was used to detect the activation of NF-κB p65 subunit in DC2.4 cells treated as above. In comparison of the effects of IL2-GMCSF and the cytokines alone or in combination, IL-2 was used at 820 IU/mL and GM-CSF at 2.5 × 103 IU/mL, corresponding to the activity of the two parts of the fusion protein at 2.5 × 103 IU/mL. blk: blank; BF: IL2-GMCSF fusion protein; 2CK: the combination of GM-CSF and IL-2. *P < 0.05 compared with the control without cytokine treatment. These experiments were repeated at least three times with similar results