Fig. 1

Identification of cell receptor expression and assays of the IL2-GMCSF bioactivity. a–b qRT-PCR was used to detect the IL-2Rα and GM-CSFRα chain expression in different cell lines; c IL2-GMCSF harbored the activities of its component cytokines, as demonstrated by cell proliferation assays of mouse splenocytes for IL-2 acivity and FDC-P1 cells for GM-CSF activity; d flow cytometry assays showed that IL2-GMCSF could bind on A1.1 cells (IL-2R⁺) and DC2.4 cells (GM-CSFR⁺), but almost not on WEHI-3 cells which was used as the IL-2R⁻GM-CSFR⁻ control. These experiments were repeated at least three times with similar results