Fig. 5

Analysis of protein expression by western blot. Proteins were extracted from VX2 tumors and from normal rabbit liver cells as a control. a β-actin expression was used as reference level. VX2 tumors expressed very high levels of stemness marker (CD44, klf4, oct3/4, nanog and Aldh1a1) in comparison to normal liver cells. Higher expression of cell differentiation marker α-fetoprotein in VX2 tumors in comparison to liver cells revealed that tumors from VX2 cells are well differentiated. b Quantification of protein expression in VX2 tumors and in normal liver cells