Fig. 6
FTIR microspectroscopy on tissue section from cirrhosis and hepatocellular carcinoma. a Mosaic optical image on cirrhosis from patient #37. The small area probed with SR-FTIR (red rectangle) has been magnified several times (b) to investigate chemical composition at high spatial resolution (c). d, e Chemical images obtained mapping whole specimen with globar-FTIR. Contour plots represent the quantitative 2D distribution of collagen (d) and lipids (e) obtained integrating IR signals within the 1300–1200 cm−1 and the 3000–2800 cm−1 spectral domains, respectively (blue low; red high). f, h PC score plots (each point represents one spectrum) and g, i the corresponding average spectra in samples probed with SR-FTIR (f, g) and globar-FTIR (h, i), respectively