Fig. 5

hD1R facilitated donor cell engraftment after BM transplantation. a NOD/SCID mice were sublethally irradiated and transplanted with 5 × 10⁴ human UCB CD34⁺ cells. From the day of BM transplantation, mice were daily injected i.p. with PBS or hD1R for 7 days. On day 14 after irradiation, histology of the femurs of mice was examined by using H&E staining. b On the 14th day after BM transplantation, BM cells were collected and counted (n = 10). c Four weeks after the transplantation, the peripheral blood cells of the recipient mice were analyzed by FACS, and the percentages of engrafted human CD45⁺ cells were shown (n = 8). d Representative FACS analysis of CD45⁺ cell engraftment in (c). e BM cells from the recipient mice in (a) were subjected to CFU assay (n = 4). Bars = mean ± SD. *P < 0.05, **P < 0.01