Fig. 7

Coomassie-blue stained gels of 2D-resolved proteins from five non-tumour tissues adjacent to the CC livers. Each extract was tested with the serum of the corresponding patient (C, E, K, P and S). Arrows (CN, EN, KN, PN and SN) indicate the immunoreactive proteins stained only by CC sera. They are listed in Additional file 2: Table S2. As for their non-tumour counterparts, a widespread immunopattern was noted. Fifty-eight different proteins were identified. Fourteen proteins were selectively stained by more than three (30 %) of the CC sera (Table 1). Fructose-bisphosphate aldolase B was identified by four (80 %) of the patient sera, HSP60, prelamine A/C and serum albumin by three (60 %). Ten proteins were targets for two (40 %) of the CC sera: 3-ketoacyl-CoA thiolase, α-enolase, β-enolase, acetyl-CoA acetyltransferase, ATP synthase subunit β, catalase, epoxyde hydrolase, liver arginase, liver carboxylesterase 1 and retinal dehydrogenase