Fig. 4

Up-regulated CCL20 expression after HERC5 siRNA knockdown in HCC cell lines. a qRT-PCR analysis of HERC5 mRNA expression in three HCC cell lines 96 h after transfection of negative control siRNA and HERC5 siRNA. Shown is relative expression as copy numbers per 10⁶ endogenous control genes (average of expression levels of ACTB, GAPDH, and UBC); b qRT-PCR analysis of CCL20 mRNA expression in three HCC cell lines 96 h after transfection of negative control siRNA and HERC5 siRNA; c Secreted CCL20 in HCC cell culture medium was measured by ELISA 96 h after siRNA transfection. Standard deviations are represented for each bar