Fig. 7
Role of β-catenin in the anti-glioblastoma activities of β-elemene. G1, G2 or U87 cells were treated with 50 µg/ml β-elemene in the presence or absence of XAV939 for 24 h. a Cell viability was determined using CCK-8 assay. Treatment with XAV939 increased the anti-proliferative effect of β-elemene. b Treatment with 10 µM XAV939 markedly downregulated the expression of β-catenin and upregulated the expression of E-cadherin, GFAP, Notch1 and vimentin as shown by Western blot analysis. c The results of (b) were estimated using the Gel-Pro Analyzer 4.0 software and are illustrated graphically. The results are representative of three independent experiments, and the values are presented as the mean ± SD (*p < 0.05, **p < 0.01)