Fig. 4
From: Early outgrowth cells versus endothelial colony forming cells functions in platelet aggregation
Expression of NOS and COX and release of NO and PGI2 by EOCs and ECFCs. a Left: NOS and COX protein expression profile in PBMCs, EOCs, ECFCs and HUVECs. Representative blots of cell lysates analyzed for eNOS, iNOS, COX-1 and COX-2 by SDS-PAGE. Right: Histogram represents the mean of data ± SEM expressed as arbitrary units of optical density of blots on the left (n ≥ 3); (b, c) supernatants from 4 × 106/mL PBMCs, EOCs, ECFCs and HUVECs were assessed for PGI2 release by radioimmunoassay of 6-keto-PGF1α concentration (pg/mL) (b) and for NO release by nitrate/nitrite fluorometric assay for nitrate concentration (pmol) (c). Histograms represent the mean concentration ± SEM of nitrate and PGI2 (n ≥ 3, *p < 0.05 vs PBMCs, ***p < 0.001 vs PBMCs)