Fig. 1From: Mechanical fibrinogen-depletion supports heparin-free mesenchymal stem cell propagation in human platelet lysateProduction of standard medium (condition A), fibrinogen-depleted medium without (condition B) and with heparin (condition C). To prepare standard medium A (white), αMEM was supplemented with 10 % pooled human platelet lysate (pHPL), 5.5 mM Dipeptiven and 2 IU/mL heparin. Medium B (grey) and C (black) were prepared by supplementing αMEM with 10 % pHPL and 5.5 mM Dipeptiven and by allowing clot formation over night (o/n) followed by mechanical disruption of the fibrin gel by vigorous shaking. After removal of the fibrin pellet medium B was ready to use, while medium C was additionally supplemented with 2 IU/mL heparinBack to article page