Fig. 6

Ubiquitination of Nrf2 was regulated by miR-29/Keap1 axis in high glucose triggered HK-2 cells. a HK-2^{miR−29mimic} cells were transfected with Flag-ubiquitin and myc-Keap1 for 2 h and exposed to 5.5 and 45 mM glucose for 48 h; expression of ubiquitinated Keap1 protein was determined using western blot. b Western blot was performed to analyze expression of Keap1 and nuclear Nrf2 in 5.5 mM and 45 mM glucose-triggered HK-2^{miR−29mimic} cells