Fig. 4

a In vivo imaging of representative control, DRA-challenged and DRA-challenged and budesonide-treated mice injected with Prosense680 after 8 weeks of DRA. b Fluorescence signal quantification measured by FMT in ProSense680-injected mice at different time points after beginning of DRA, in the absence or presence of budesonide treatment beginning after 4 weeks of DRA. Values are expressed as mean ± SEM of three different experiments. The dashed line represents the average value of control animals treated with saline. c Linear regression between the intensity of fluorescence resulting from ProSense680 and the total number of cell in BAL fluids from control, DRA-challenged and DRA-challenged and budesonide-treated mice injected with Prosense680 after 8 weeks of DRA. Eight mice were used for every time points and the experiment was replicated three times. Values are expressed as mean ± SEM of the three different experiments. Statistical differences were tested by one-way ANOVA followed by Dunnett’s t post hoc test for group comparisons. P < 0.05 and **P < 0.01