Fig. 2

a Number of monocytes/macrophages in BALF from control and DRA challenged mice treated with either saline or budesonide at different time points. Values are expressed as number of cells per μL as measured by Dasit Sysmec XT 1800. The dashed line represents the average value of control animals treated with saline. b Expression of CD11b integrin surface marker on monocytes/macrophages present in BALFs as measured by FACS analysis in BALF from DRA challenged mice treated with either saline or budesonide at different time points. Values are expressed as Mean Fluorescence Intensity (MFI). The dashed line represents the average value of control animals treated with saline. c Representative picture of CD11b integrin surface marker on monocytes measured by FACS analysis; CD11b expression of mice treated with either saline (dark grey) or 8 weeks of DRA (black), with isotype control being depicted in light grey. d Representative picture of CD11b integrin surface marker on monocytes measured by FACS analysis; CD11b expression of mice treated with either saline (dark grey) or 8 weeks of DRA and budesonide beginning after 4 weeks of DRA (black), with isotype control being depicted in light grey. Eight mice were used for every time points and the experiment was replicated three times. Values are expressed as mean ± SEM of the three different experiments. Statistical differences were tested by one-way ANOVA followed by Dunnett’s t post hoc test for group comparisons. *P < 0.05 and **P < 0.01 vs time-matched DRA group