Fig. 3
Inflammation and cofilin1 activity induced by Angiotensin II in the cultured HK-2 cells. HK-2 cells were pre-incubated with or without GSPE (50 μg mL−1) for 12 h and then treated with AngII 10−6mol l L−1 for 12 h. a Then total cell lysates were immunoblotted with anti-IL-1β, anti-MCP1, anti-p-cofilin1 and anti-cofilin1 antibodies. b Quantification of p-cofilin1 level normalized to cofilin1 in a, c quantification of IL-1β, MCP1, cofilin1 levels in a. Values are mean ± SD, *P < 0.01 vs. control group, #P < 0.05, ##P < 0.01 vs. GSPE + AngII treated group