Fig. 3

PEP-1-MsrA inhibited oxidation, cell death and inflammation in macrophages. a Intracellular ROS levels were measured by flow cytometry. RAW264.7 macrophages were pretreated with PEP-1-MsrA or MsrA (1 µM) and stimulated by 1 mM H₂O₂ for 1 h. b The apoptosis of mouse peritoneal macrophages induced by H₂O₂ was quantified by flow cytometry. c–e The mRNA levels of inflammation-associated cytokines IL-1β, TNFα and IL-10 were determined by q-PCR. Mouse peritoneal macrophages were treated with 25 ng/ml LPS, and 1 µM PEP-1-MsrA or MsrA for 3 h before mRNA was extracted. n = 3–4 for each group, *P < 0.05, **P < 0.01 vs control group. ^# P < 0.05, ^## P < 0.01 vs LPS-induced group. ^Δ P < 0.05, ^ΔΔ P < 0.01 vs MsrA-treated group