Fig. 5

YangZheng XiaoJi had an inhibitory effect on the activation of the HGF receptor, cMET in lung cancer cell, A549. Top images from immunoblot assay. The phospho-MET (pMET), total MET (cMET) and the loading control GAPDH were probed with the respective antibodies. Bottom the pMET/cMET band density ratio for the immunoblots. The medicinal extract, DME25 was used at 1:1000 and cMET small inhibitor PHA665752 at 10 nM. Positive control for protein tyrosine phosphorylation was sodium orthovanadate (100 µM) with hydrogen peroxide (0.1 %). In quiescent cells, the limited activation of pMET was marked reduced by DME25, PHA665752 and the combination as indicated by asterisk. HGF caused marked action of the receptor (p < 0.01). The HGF induced activation was significantly inhibited by the DME25/PHA665752 combination (asterisk), however only marginally by DME25 alone (^ap = 0.13 vs with HGF only) and PHA665752 (^bp = 0.14, vs with HGF only)