Fig. 4

Exogenous expression of miR-101 reduces cell proliferation and induces cell cycle arrest in Hep-2 cells. A MiR-101 expression significantly increased in the miR-101-treated group compared with that in the negative control group and the blank control group in real-time RT-PCR assays (* P < 0.05). B Cell proliferation was reduced in the miR-101-treated group compared with the control groups 48, 72, and 96 h post-transduction (P < 0.05). C Cell cycle profiles are shown for: Hep-2 cells in the blank control group (a) [G0/G1 = (64 ± 2.16) %, S = (28.94 ± 2.30) %, G2/M = (7.06 ± 1.40) %]; Hep-2 cells in the negative control group (b) [G0/G1 = (65.11 ± 1.05) %, S = (28.53 ± 1.14) %, G2/M = (6.36 ± 1.33) %]; and Hep-2 cells in the miR-101-treated group (c) [G0/G1 = (73.79 ± 2.45) %, S = (22.73 ± 1.05) %, G2/M = (3.47 ± 2.27) %].