Fig. 3

MiR-101 directly targets the 3′ UTR of CDK8 mRNA. a Wildtype and mutated miR-101 target sites in the 3′ UTR of CDK8 were cloned into luciferase reporter vectors. b Luciferase activity for the wildtype CDK8 reporter significantly decreased by 43 % in HEK293T cells that expressed miR-101 compared with the control cells (*P < 0.05). c Luciferase reporter gene assay for measuring interactions between miR-101 and 3′-UTR of the CDK8 mutant in HEK293T cells. There was no significant difference between groups (P > 0.05). The luciferase experiments were repeated three times.