Fig. 6

Treatment with rhACE2 abolished Ang II-mediated reduction in renal nephrin expression in ApoEKO mice. a Representative Western blot analysis showing ACE2 protein levels in the mice kidneys. b, c The Ang II and Ang-(1-7) levels (b) and the ratio of Ang-(1-7)/Ang II (c) in the kidney cortex of the ApoEKO mice treated with Ang II and/or human recombinant ACE2 (rhACE2). d, e The real-time PCR (d) and Western blotting analyses (e) exhibited levels of nephrin in mice kidneys. β-actin or GAPDH was used as an endogenous control. n = 5–6; RE relative expression, AU arbitrary units; **P < 0.01 compared with ApoEKO control group; ^# P < 0.05; ^## P < 0.01 compared with ApoEKO + Ang II group.