Fig. 4

In vivo imaging of lung inflammation induced by P. aeruginosa culture supernatants on IL-8 transiently transgenic mice. a Representative images of mice (n = 3 per group) transiently transgenized with bIL-8-Luc and intratracheally instilled with bacterial cell-free 1X, 3X, 10X and 30X supernatants from VR1. The growth medium TSB was used as a control. Mice were monitored at 4, 24 and 48 h post stimulation by BLI drawing a region of interest (ROI) over the chest. b Representative images of mice (n = 8 per group) transiently transgenized with bIL-8-Luc and intratracheally instilled with bacterial cell-free, 10X supernatants from VR1 and VR2 strains grown in presence or absence of AZM (VR1 ± AZM and VR2 ± AZM). The growth medium TSB was used as a control. Mice were monitored at 4, 24 and 48 h post stimulation by BLI drawing a region of interest (ROI) over the chest. Data are also presented as light intensity quantification of the ROI using the LivingImage software. The experiment was repeated 3 times and each point represents the mean ± standard error of 8 animals. Data were expressed as FOI over baseline activity of each mice and statistical differences were tested by one way ANOVA followed by Dunnett’s post hoc test for group comparisons. Results are reported as mean ± SEM and significance attributed when P < 0.05 (*) or P < 0.01 (**).