HPV-16 E6/E7 suppression reduced cell proliferation and increased apoptosis. a and b SiHa and CaSki cells were transfected with various PLAP promoter/enhancer driven test/control shRNAs in different doses and percent cell proliferation was evaluated by MTT assay on the 6th day. Decrease in cell proliferation was dependent on the dose and strength of the shRNA construct (p < 0.05). Also, NFκBEn–Pr+2-HPV-16–E6/E7, when compared to PLAPPr+2-HPV-16–E6/E7, significantly reduced cell proliferation of both SiHa and CaSki to a greater degree (p = 0.042 and p = 0.029, respectively). c and d Flow cytometry was used to evaluate apoptosis (subG1). Increase in apoptosis was concordant with the strength of promoter/enhancer construct and corroborated with the cell proliferation studies.