Fig. 3

The effect of SPRY4-IT1 on gastric cancer cells proliferation in vitro and vivo. a MTT assay was performed to determine the proliferation of pCDNA-SPRY4-IT1 or si-SPRY4-IT1 transfected BGC823 and SGC7901 cells. Data represent the mean ± SD from three independent experiments. b Colony-forming growth assay was performed to determine the colony formation ability of pCDNA-SPRY4-IT1 transfected BGC823 and SGC7901 cells. The colonies were counted and captured. c The tumor volume was calculated every 3 days after injection of BGC823 cells stably transfected with pCDNA-SPRY4-IT1 or empty vector. d Tumor weights are represented as means of tumor weights ± SD. e Tumors developed from pCDNA-SPRY4-IT1 transfected BGC823 cells showed lower ki67 protein levels than tumors developed by control cells. Upper H & E staining; lower: immunostaining. *P < 0.05 and **P < 0.01.