Figure 2

Characterization of rabbit MSCs. A Morphology of bone marrow and adipose-derived mesenchymal stromal cells obtained from one rabbit. MSCs from both sources display the characteristic spindle-shaped morphology. Magnification ×4. B Immunophenotype of culture-expanded ASCs obtained from one representative rabbit. ASCs were positive for CD90, CD29 and CD49e and negative for CD45 and CD10, as reported [20]. The immunophenotype of BM-MSCs was superimposable. C Osteogenic and adipogenic differentiation capacity of BM-MSCs and ASC. Differentiation into osteoblasts was demonstrated by the histological detection of Alkaline phosphatase activity (a) and calcium depositions positive for Alizarin Red (b); magnification ×20. Differentiation into adipocytes was revealed by the formation of lipid droplets stained with Oil Red O (c); magnification ×20. Non differentiated cells (negative ctrl) are reported.