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Figure 6 | Journal of Translational Medicine

Figure 6

From: Anthocyanin-rich Seoritae extract ameliorates renal lipotoxicity via activation of AMP-activated protein kinase in diabetic mice

Figure 6

Effect of AMPKα1, AMPKα2, and SIRT1 siRNAs on anthocyanin-stimulated AMPK signalling in HGECs. Protein lysates (10 μg) from cultured HGECs were separated by SDS-PAGE and analysed by western blotting. Cultured HGECs were transfected with 40 nmol/L control siRNA or 40 nmol/L AMPKα1, AMPKα2, or SIRT1 siRNAs using a transfection reagent (Lipofectamine 2000). At approximately 24 h after transfection, the levels of phospho-AMPK Thr172, total AMPK, SIRT1, and PGC-1α signalling in low-glucose medium were analysed. ad Representative western blots (a) and quantitative analyses of the results bd. *p < 0.05 and **p < 0.01 compared with the other groups. Cultured HGECs were transfected with 50 nmol/L control siRNA or 50 nmol/L AMPKΑ1, AMPKΑ2, or SIRT1 siRNAs and stimulated with anthocyanin in high-glucose medium. At approximately 24 h after stimulation, the levels of phospho-AMPK Thr172, total AMPK, SIRT1, PPARα, PGC-1α, and ERR-1α signalling in high-glucose medium were analysed. ej Representative western blots of phospho-AMPK Thr172, total AMPK, PPARα, PGC-1α, ERR-1α, and β-actin levels (e) and quantitative analyses of the results (fj). *p < 0.05 and **p < 0.01 compared with high-glucose medium. HG high glucose.

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