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Figure 5 | Journal of Translational Medicine

Figure 5

From: Anthocyanin-rich Seoritae extract ameliorates renal lipotoxicity via activation of AMP-activated protein kinase in diabetic mice

Figure 5

Effect of anthocyanins on intracellular signalling, apoptosis, and oxidative stress in HGECs cultured in low-glucose (5 mmol/L d-glucose) or high-glucose (40 mmol/L d-glucose) medium with or without anthocyanin treatment (50 μg/mL). Phospho-Thr172 AMPK, total AMPK, PPARα, PGC-1α, ERR-1α, PPARγ, phospho-ACC, total ACC, and β-actin levels were assessed using cultured HGECs. Protein lysates (10 μg) were separated by SDS-PAGE and analysed by western blotting. a Representative western blots and quantitative analyses of phospho-Thr172 AMPK, total AMPK, PPARα, PGC-1α, ERR-1α, and β-actin. be Quantitative analyses of phospho-Thr172 AMPK/total AMPK (b), PPARα (c), PGC-1α (d), and ERR-1α (e). fh Representative western blots of PPARγ, phospho-ACC, total ACC, and β-actin in HGECs (f) and quantitative analyses of the results g, h. il Representative western blots of SOD-1, SOD-2, BCL-2, BAX, and β-actin levels in HGECs (i) and quantitative analyses of the results (jl). *p < 0.05 and **p < 0.01 compared with high-glucose medium. HG high glucose, LG low glucose.

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