Figure 3

Anti-apoptotic BCL-2, pro-apoptotic BAX, and TUNEL assays in the renal cortex of db/m and db/db mice with or without anthocyanin treatment. Protein lysates (10 μg) from the renal cortex were separated by SDS-PAGE and analysed by western blotting. a Representative western blots of BCL-2, BAX, and β-actin levels. b Quantitative analyses of the BCL-2/BAX ratio. c–g Representative immunohistochemical staining for TUNEL-positive cells in db/m control (c), db/m ATC (d), db/db control (e), and db/db ATC (f) mice, and quantitative analyses of the results (g). **p < 0.01 vs. db/m control, db/m ATC, and db/db ATC mice.