Figure 3

Myeloma patients occasionally develop spontaneous IgG antibody responses against SLLP1. Based on the unavailability of recombinant full-length SLLP1 protein with a sufficient quality, we decided to use SLLP1 20-mer peptides overlapping by 10 amino acids for the detection of spontaneous anti-SLLP1 IgG antibody responses. a For the ELISA assays, plates were coated with two pools of peptides containing SLLP1 peptides 1–10 (pool SLLP1-1; left) and 11–21 (pool SLLP1-2; right), respectively, Dots indicate intensity of the signal as measured by an absorbance reader. b Target epitopes of the SLLP1-specific antibody response of MM patient UKE-164 were identified using single overlapping 20-mer peptides spanning the complete sequence of SLLP1 in an ELISA assay. Recombinant influenza nucleoprotein (NP) and tetanus toxoid (TT) were used as positive controls, recombinant SSX-2 protein served as an irrelevant control protein. Bars indicate intensity (mean + SEM) of the signal against the given epitope. All results were validated by three independent experiments.