Figure 5

Attachment of cardiac cells on scaffolds. False colored scanning electron microscope images of human cardiomyocytes on human heart extracellular matrix scaffolds in a high, b medium, and c low magnifications. d Two photon microscope image of a scaffold overlaid over a murine heart. The second harmonic of the collagen fibrils are visualized. Dissecting microscope images (representative) of a human cardiomyocyte-seeded human ECM scaffold on an adult mouse heart: e shows scaffold in brightfield, f shows autofluorescent scaffold on a lesser autofluorescent mouse heart, and g shows red florescent cardiomyocytes within the scaffold. Scaffolds spontaneously fused to mouse heart within 3 min.