Figure 6

Quantitative RT-PCR analysis of inflammatory mediators in the renal tissues of severely burned rats. The qRT-PCR analysis revealed that HS treatment could significantly attenuate the clear elevation in mRNA expression after burn in the rat kidneys (a–c, e). Additionally, positive IHC results for IL-10 were observed post burn, and more positive renal cells were identified with HS treatment at various time points (d). Although the expression of IL-10 mRNA markedly increased with time, HS treatment appeared to enhance this increase in the kidneys of burned rats (d). The sample size was n = 6 for each group. The results are expressed as the means ± SEMs. *p < 0.05, **p < 0.01, vs Burn + saline; ^#p < 0.05, ^##p < 0.01, vs Sham.