Figure 8

TGF-β expression in the sections of LLABCs, using IHC staining, at ×200 magnification. Briefly, heat-mediated antigen retrieval was performed using citrate buffer, pH 6 (20 min). The sections were then incubated with MAbs to TGF-β (Abcam, ab64715) at a concentration of 12 µg/ml for overnight at 4°C. Polymeric HRP-linker antibody conjugate was used as secondary antibody. DAB chromogen was used to visualize the staining. The sections were counterstained with haematoxylin. a Low level of TGF-β expression, b high level of TGF-β expression. The H score [% of positive cells × intensity of staining (1–3)] was used to assess the level of expression; sections were scored as negative or positive. Scoring performed on whole tissue section (>10 HPFs). Itu intratumoural, Str peritumoural.