Figure 2

The effect of miR-409-3p on the proliferation of CRC cells. a Cell proliferation was measured by the WST assay. SW480 cells were transfected with miR-409-3p mimics or NC mimics at a final concentration of 100 nM and, at 24 h post-transfection, the WST assay was performed every 24 h for 4 days. Results aremeans of three independent experiments ± SD (**P < 0.01). b SW1116 cells were transfected with miR-409-3p mimics or NC mimics at a final concentration of 100 nM and, at 24 h post-transfection, the WST assay was performed every 24 h for 4 days. Results are means of three independent experiments ± SD. c The effect of miR-409-3p on cell proliferation was evaluated by the plate colony formation assay. SW480 cells were transfected with miR-409-3p mimics or NC mimics then seeded onto 6-well plates. The number of colonies was counted on the 14th day after seeding. d SW1116 cells were transfected with miR-409-3p mimics or NC mimics then seeded onto 6-well plates. The number of colonies was counted on the 14th day after seeding. e, f Colonies containing 50 or more cells were counted. Results are means of three independent experiments ± SD (*P < 0.05).