Table 1 Primer and probe sequences used for quantitative reverse transcription-polymerase chain reaction (qRT-PCR)
Gene symbol | NM_number | Probe name | Probe sequence | Forward name | Forward sequence | Reverse name | Reverse sequence |
|---|---|---|---|---|---|---|---|
EGFR | NM_201283 | MP483 | CCTTGCCGCAAAGTGTGTAACGGAAT | MP483_For | CGCAAGTGTAAGAAGTGCGAA | MP483_Rev | CGTAGCATTTATGGAGAGTGAGTCT |
H2N (HER2) | M11730_gen | MP452 | AGGCCAAGTCCGCAGAAGCCCT | MP452_For | TCTGGACGTGCCAGTGTGAA | MP452_Rev | CCTGCTCCCTGAGGACACAT |
HER3 | NM_001982 | MP489 | CTCAAAGGTACTCCCTCCTCCCGGG | MP489_For | CGGTTATGTCATGCCAGATACAC | MP489_Rev | GAACTGAGACCCACTGAAGAAAGG |
HER4 | NM_005235 | MP086 | CACAGACTGCTTTGCCTGCATGAATTTC | MP086_For | GAGGCTGCTCAGGACCTAAGG | MP086_Rev | GAGTAACACATGCTCCACTGTCATT |
CALM2 | NM_001743 | MP501 | TCGCGTCTCGGAAACCGGTAGC | MP501_For | GAGCGAGCTGAGTGGTTGTG | MP501_Rev | AGTCAGTTGGTCAGCCATGCT |