Fig. 3

In vitro cytokine release in response to T7-OCT4 conjugates. Total murine spleen lymphocytes were isolated and treated with T7 agonist or T7 conjugates at various concentrations as indicated. Culture supernatants were harvested 24 h later, and cytokine levels were measured by immunoassay. The results are a representative of at least two separate experiments in triplicate per treatment. (a and b) Levels of IL-12 and IFN-Ƴ released by spleen lymphocytes treated with T7 agonist, M2e peptide and T7-M2e conjugate. (c and d) Levels of IL-12 and IFN-Ƴ released by spleen lymphocytes treated with T7 agonist, MG7 peptide and T7-MG7conjugate. e and f: Levels of IL-12 and IFN-Ƴ released by spleen lymphocytes treated with PBS, T7 agonist, OCT4 protein, T7 + OCT4 mixture and T7-OCT4 conjugate. Values are the means ± SD, n = 4; *, P < 0.01 vs Control;#, P < 0.01 vs OCT4; Δ,P < 0.01 vs T7 + OCT4. (Note: The concentration in the Figure describes the molarity of T7 agonist. The molarity ratio of OCT4 protein: T7 agonist = 1:4)