Fig. 1

The mRNA and protein expression of VEGF111b could be induced by Mitomycin C in ovarian cancer cells. (a) RT-PCR to detect VEGF111b mRNA. After treating with 100 μg/ml mitomycin C, VEGF111b mRNA was observed in ovarian cancer cells SKOV3 (line 1) and OVCAR3 (line 3). But VEGF111b mRNA was not observed in the cells (line 2 and line 4). (b) VEGF111b protein was detected in SKOV3 cells (B, line 1) and OVCAR3 cells (line 3) after treating with 100 μg/ml mitomycin C by using VEGF111b polyclonal antibody, but not observed in mitomycin C-unexposed SKOV3 and OVCAR3 cells (line 2 and line 4). (c) In VEGF111b and VEGF165b group, VEGF111b and VEGF165b protein were observed in the conditioned mediums and lysates in the OVCAR3 cells transfected with VEGF111b or VEGF165b using lentivirus