Fig. 4

CAR^hi phenotype does not require high genomic copy number of CAR transgene. a Relative genomic CAR copy number in three sorted CAR–T-cell populations as determined by quantitative PCR relative to the housekeeping gene β-actin. The lowest copy number is set to 1. Values are averages of quadruplicates with error bars indicating ± 1 s.d. b CAR–T-cell populations with varying genomic CAR copy number separate into CAR^hi/CD25⁺ and CAR^lo/CD25⁻ populations within 18 h of antigen simulation