Fig. 2

In vitro expansion of isolated CMV-CTL and proliferative capacity of expanded cells. CMV-CTL obtained from G-CSF mobilized (n = 3) and non-mobilized (n = 3) samples were expanded in vitro during 21 days co-cultured with irradiated CMV-loaded feeders and homeostatic cytokines. Viable cells were counted every 2–3 days and fold expansion was calculated related to the cells at the beginning of the culture. Represented percentages are obtained from CD3+ T cell gate. (a) Mean fold expansion of CMV-CTL obtained from G-CSF mobilized vs non-mobilized PBMCs. Mean percentages of CD3 + CD8 + ST+ cells (b) and CD69+ or CD137+ cells (c) during culture