Fig. 1
CMV-CTL isolation by MHC-Streptamers. CMV-CTLs, that specifically recognize the CMVpp65495–503 peptide of the CMV, were sorted using ST from G-CSF mobilized (n = 3) and non-mobilized (n = 3) donor samples. (a) Mean yield and purity of ST+ cell selection process from original G-CSF mobilized and non-mobilized PBMCs. (b) Representative figure of CD3 + CD8 + ST+ cell isolation. ST expression is represented in resting PBMCs and in the positive fraction. Displayed cells were previously gated on CD3+ T cells