Detection and quantification of microRNA in cerebral microdialysate

Table 2 Criteria for categorization

Groups	Inclusion criteria for each specific miRNA	Based on samples	miRNA targets
High RR	in vitro Cq_{mean CSF} – Cq_{mean MD} < ±2 and in vitro SD_CSF and SD_MD both < 1	6-13	63
High RR		6-13	63
Low RR	in vitro Cq_{mean MD} - Cq_{mean CSF} > 4 and in vitro SD_CSF < 1	6-13	53
Low RR		6-13	53
Indetermined RR	Criteria not fulfilled	6-13	173
Not present	Not detected in any samples	6-13	465

Criteria for categorization of 754 screened miRNAs according to their relative recovery in microdialysate from a catheter with a membrane cut-off value of 20 kDa.
RR, relative recovery; Cq_{mean CSF}, mean cycle quantification of the specific miRNA in sample 10-13, Table 1; Cq_{mean MD}, mean cycle quantification of the specific miRNA in sample 6-9, Table 1; SD_CSF, standard deviation of cycle quantification of the specific miRNA in sample 10-13, Table 1; SD_MD, standard deviation of cycle quantification of the specific miRNA in sample 6-9, Table 1.

ISSN: 1479-5876