Figure 5

Kinetics of phenotypic and functional exhaustion profile depending on the nature of the stimulating antigen. (A) CD8⁺ LMP2_426-434 ⁺ cells expressing PD-1 and KLRG-1 in both culture steps over time; n = 3, data represented as average ± SEM. (B) CD8⁺ T cells at each culture time points were analysed by flow cytometry for intracellular staining of Ki67; left: one representative and right: n = 3, data represented as average ± SEM. (C) Intracellular staining analysis of TNFα, IFNγ and IL-2 production after LMP2_426-434 peptide restimulation at day 12 and 21 of the REP.