Figure 2

E2-induced HOTAIR increases the migration of breast cancer cells. A): MDA-MB-231 and BT549 cells were treated with the indicated concentration of E2 for 24 hours and the expression of HOTAIR was assessed using real-time PCR. B): MDA-MB-231 and BT549 cells were treated with 10 nM E2 for varying hours and the expression of HOTAIR was assessed using real-time PCR. C): MDA-MB-231 and BT549 cells were treated with 10 nM E2 for varying hours and the migrated cells were counted. D): siHOTAIR RNA sequences (si-HOTAIR1, si-HOTAIR2 and si-HOTAIR3) were transfected into MDA-MB-231 for 24 h, and then the HOTAIR level was detected by using qRT-PCR. E): MDA-MB-231 and BT549 cells were transfected with the control sequence or siHOTAIR RNA sequences before treatment with 10 Nm E2 for 36 hours and the migrated cells were counted. The results are shown as mean ± S.E. from three representative independent experiments. *p < 0.05, **P < 0.01 compared with 0 h or control. ^#p < 0.05 The results are shown as mean ± S.E. from three representative independent experiments. **P < 0.01 compared with control. ^#p < 0.05 compared with E2.