Figure 6

Effect of overexpressed DNMT1 on cisplatin sensitivity of A549/DDP cells. A549/DDP cells transfected with pcDNA3.1-DNMT1 or pcDNA3.1 were used in experiments. (A) Expression of DNMT1 was evaluated using western blot assay. (B) After transfected with miR-148b mimic for 24 h, cells were incubated with various doses of cisplatin (10, 20, 40, 80, 160, 320 μmol/L), and then cell viability was assessed using MTT assay. (C) After transfected with miR-148b mimic24 for 24 h, cells were incubated with 10 μmil/L cisplatin, and then cell apoptosis was determined using flow cytometry. Data were represented as mean ± SD. *P <0.05 compared with NC; #P <0.05 compared with miR-148b mimic.