Figure 4

Targeting site of miR-148b in the DNMT1 3′UTR. (A) DNMT1 3′UTR was predicted a binding site for hsa-miR-148b. The mouse DNMT1 mRNA sequence is shown with potential binding sites indicated in white. The highly conserved mature miR-148b sequence in mammals and potential binding between the miR-148b seed region to the mouse DNMT1 3′UTR sequence are shown. (B) After A549/DDP transfected with miR-148b mimic for 24 h, binding of miR-148b and DNMT1 was assessed by Luciferase reporter assays and expression of DNMT1 mRNA was assessed using Q-RT-PCR. (C) After A549 transfected with miR-148b ainhibitor for 24 h, binding of miR-148b and DNMT1 was assessed by Luciferase reporter assays and expression of DNMT1 mRNA was assessed using Q-RT-PCR. Data were represented as mean ± SD. *P <0.05 compared with NC