Figure 9

Metabolic characterization of undifferentiated and differentiated SGBS cells. Where noted rosiglitazone (rosi) was added during the first initial days of differentiation at a final concentration of 2 μM. (a) SGBS respiratory complexes expression. An overall increase in expression results from differentiation in the presence of rosiglitazone (*p < 0.02). Values are presented as the fluorescent units ratio between each respiratory complex protein and the nuclear encoded NNT protein ± SEM. (b) Oxygen consumption rate (OCR) by SGBS pre-adipocytes and adipocytes in unbuffered DMEM containing 5 mM pyruvate and 2.5 mM glucose. Basal and FCCP-dependent State3u respiration peaked at D14 in a rosi-dependent manner. (c) Basal, uncoupled and maximal respiration are shown. Basal respiration is increased in adipocytes at D14 in the presence of rosi (*p < 0.05 vs. adipocyte D14 – rosi; & p < 0.01 vs. pre-adipocyte). Maximal respiratory capacity is increased in the adipocytes differentiated in the presence of rosi at D14 and D28 (*** p < 0.0001 vs. pre-adipocyte, # p <0.0001 vs. adipocyte D14 or D28– rosi; @ p < 0.0001 vs adipocyte D28 + rosi). Results are expressed as mean ± SEM.