Figure 10

Metabolic characterization of undifferentiated and differentiated SW872 cells. (a) SW872 respiratory complexes expression. Differentiation in oleate increases the expression of the mitochondrial respiratory complexes. Values are presented as the fluorescent units ratio between each respiratory complex protein and the nuclear encoded NNT protein ± SEM. (b) OCR by SW872 pre-adipocytes and adipocytes in unbuffered DMEM containing 5 mM pyruvate and 2.5 mM glucose. Basal and FCCP-dependent State3u respiration are increased in the RA-treated adipocyte relative to the pre-adipocyte state (** p < 0.001; ***p <0.0001 versus pre-adipocyte; # p <0.05 vs. adipocyte + RA + T₃ + forskolin/IBMX)). (c) Basal, uncoupled and maximal respiration are calculated. RA increased the maximal respiratory capacity of the adipocytes relative to the pre-adipocytes (*p < 0.05). Results expressed as mean ± SEM.