Figure 1

Human brown PAZ6 adipocytes accumulate multiple small lipid droplets upon differentiation and express peri-nuclear and cytoplasmatic UCP1 at high levels. PAZ6 cells were differentiated for 14 days and cell monolayers were stained and examined by microscopy. (a) Oil Red staining was carried out as described above and the presence of stained lipid droplets at D7 (left) and D14 (right) was assessed at a magnification of 20×. (b and c) Premature and differentiated PAZ6 cells were co-stained with mitotracker (green) to test the abundance of mitochondria, lipidtox (red, in b) for neutral lipid droplets or anti-UCP1 antibodies (red, in c) and DAPI (blue) to visualize nuclei. Single-channel images were overlayed and processed by Photoshop software. All scale bars are reported.