Figure 2

Morphology of primary cell lines and Hedgehog ligand expression A) Phase contrast images of cell lines generated from xenografts. B) H&E stained tumor grown from orthotopically injected PC053M PDAC cells (right panel) compared to p1 PDX tumor (left panel). C) As for panel using subcutaneous injections of EAC007B, compared to p2 PDX. D) Hh ligand expression determined by qPCR of respective cell lines. E) Surface expression of Hh protein on primary lines measured by flow cytometry using 5E1.