EMT phenotype in radioresistant esophageal cancer cells. Reduced expression of E-cadherin and increased expression of Vimentin, Slug, Twist and Snail were found in KYSE-150R cells. (A) EMT makers genes expression determined by reverse transcription polymerase chain reaction (RT-PCR). All samples were normalized to the signal generated from housekeeping gene (GAPDH). (B) Western blotting assessed E-cadherin, Vimentin and Slug in KYSE-150R group and control group. GAPDH was used as a loading control.