Localization and excretion of urinary Fetuin-A in newborn kidneys. (a) Frozen sections of WT (n = 2) and Bicc1KO newborn kidneys (n = 4) labeled with anti-Fetuin-A antibody (green), and Lotus tetragonolobus lectin (LTL, red). Bicc1 heterozygotes (n = 2) were indistinguishable from WT (not shown). Scale bars: 100 μm. (b) Western blot analysis of urinary Fetuin-A excretion of the indicated genotypes at stages P0 and P3. Signal quantification by ImageJ is shown below. Error bars represent standard deviation.