Figure 5
From: Characterization of HIV-1 entry inhibitors with broad activity against R5 and X4 viral strains
Flow cytometric analyses in 3T3 cells expressing Env (A) or CXCR4 and CCR5 (B). Three concentrations (30, 10 and 3 μM) of the ND-4043 compound were used to compete with binding of anti HIV envelope (A) or anti coreceptor (B) moAbs. Incubation with ND-4043 was carried out in PBS plus 2% of heat-inactivated FCS. DMSO refers to control cells stained with the moAbs but pre-incubated only with DMSO at 0.2%, corresponding to the concentration present in 30 μM of the compound; uns: unstained cells. A color-coded legend is displayed within the figure.