Figure 4

Hypoxia required the αvβ3 integrin pathway to drive the migration and invasion of U251 glioma cells. The expression of αvβ3 integrin was determined by immunofluorescence after treatment with melatonin (A) or integrin β3 siRNA (B) under hypoxia. All data were expressed as specific fluorescence index (SFI) values. (C) Integrin β3 siRNA treatment reduced the phosphorylation of FAK and Pyk2 in U251 glioma cells under hypoxia. (D) Integrin β3 siRNA treatment inhibited the migration and invasion of U251 glioma cells under hypoxia. Cells were stained by 0.1% crystal violet (upper) and the cell number of control was expressed as 100% (lower). *p <0.05, **p <0.01 vs control.